pcDNA6.2-GWEmGFP-miR negative

Comments for:

CMV promoter:

attB1 site:

EmGFP:

EmGFP forward sequencing primer site:

5’ miR flanking region:

miR-neg control:

3’ miR flanking region:

attB2 site （c）:

miRNA reverse sequencing primer site （c）:

TK polyadenylation signal:

f1 origin:

SV40 early promoter and origin:

EM7 promoter:

Blasticidin resistance gene:

SV40 polyadenylation signal:

pUC origin （c）:

Spectinomycin resistance gene （c）:

Spect ni omycin

5’ miR flanking

 region attB1 EmGFP attB2

pcDNATM 6.2-GW/EmGFP-miR-neg

control plasmid

5759 nucleotides

1 - 588

680 - 704

713 - 1432

1409 - 1428

1492 - 1518

1519 - 1578

1579 - 1623

1652 - 1676

1667 - 1686

1705 - 1976

2088 - 2516

2543 - 2851

2906 - 2972

2973 - 3371

3529 - 3659

3797 - 4470

4540 - 5550

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：楊建輝

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