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Mediomics公司于2001年創(chuàng)立于美國密蘇里州，Mediomics已通過ISO 9001: 2015認證并致力于為科研機構(gòu)、即時檢測、醫(yī)藥企業(yè)以及醫(yī)療和環(huán)境研究市場提供創(chuàng)新的檢測試劑盒和生物傳感器等產(chǎn)品，其檢測試劑盒可以簡單快速的定量檢測生物學和臨床相關(guān)的大分子物質(zhì)，包括DNA、生物標志物、激素、抗體、細胞內(nèi)信號分子、蛋白質(zhì)復合物、細菌、真菌和病毒等。
Mediomics代理——上海起發(fā)
Mediomics目前擁有四個核心專有技術(shù)：
1. Bridge-It：一種快速靈敏的DNA-蛋白質(zhì)結(jié)合物檢測平臺。
Bridge-It系列試劑盒可用來檢測DNA結(jié)合蛋白及其配體，和共調(diào)節(jié)因子。該系列試劑盒基于一個簡單的實驗原理：一段針對已知蛋白的具有序列特異DNA結(jié)合位點的DNA雙鏈被分為兩段，每段含有一個短的單鏈突出端。這些突出端很短，因此沒有靶蛋白時他們不會自發(fā)的重新結(jié)合。當靶蛋白出現(xiàn)時，靶蛋白與完整的DNA序列結(jié)合位點的高親和力使得兩段DNA雙鏈重新結(jié)合。通過在每段位點加入適當?shù)臒晒馓结樉涂梢詸z測到該結(jié)合。然后用熒光共振能量轉(zhuǎn)移技術(shù)（fluorescence resonance energy transfer，F(xiàn)RET）檢測熒光信號的變化來判斷DNA結(jié)合蛋白或其配體。
該系列試劑盒具有以下特色：
1)簡單的混合和測試方法：只需要混合樣本和反應試劑，不需要其他操作技術(shù)。直接檢測樣本無需稀釋步驟，只需混合樣本/標準品和反應試劑。2)快速：孵育30min后即可檢測熒光信號。3)定量檢測。4)可重復性好：實驗變異性小，避免了抗體或放射性探針帶來的批次間的差異。5)可實現(xiàn)高通量篩選。
2. PINCER：一種快速的生物傳感器、生物測定和生物芯片平臺。
PINCER Assays是一種用于定量檢測生物樣本中的生物標志物、細胞和病原體濃度的技術(shù)。該系列產(chǎn)品分為FRET-PINCER Assay和TRF-PINCER Assay兩種，分別使用熒光共振能量轉(zhuǎn)移技術(shù)（fluorescence resonance energy transfer，F(xiàn)RET）和時間分辨熒光（Time-Resolved Fluorometry，TRF）方法檢測。其檢測原理是：帶有熒光探針的抗體由一個靈活的連接物通過互補的單鏈DNA突出端與DNA雙鏈化學結(jié)合，這些突出端使得兩個DN**段有結(jié)合的可能性，然而在沒有靶蛋白的情況下，兩個DNA片段結(jié)合的可能性極小。當存在靶蛋白時，與PINCER試劑結(jié)合的靶蛋白將促使互補DNA單鏈突出端的結(jié)合而形成雙鏈DNA。這種緊密穩(wěn)定的結(jié)合使熒光團聚合起來，發(fā)生熒光共振能量轉(zhuǎn)移。因此，檢測樣本發(fā)出的熒光能量水平或亮度的變化即可定量靶蛋白的濃度。
PINCER Assay系列試劑盒的特色：
1)簡便：混合樣本/標準品與分析試劑在室溫孵育。
2)快速：孵育30min后檢測熒光信號。
3)檢測范圍廣：直接檢測樣本，無需稀釋步驟。
4)兼容性：與大多數(shù)熒光讀數(shù)儀兼容。
5)可實現(xiàn)高通量篩選。
3、使用創(chuàng)新技術(shù)篩選和研發(fā)可用于科研、臨床診斷和治療的新型高親和力捕獲試劑。
4、抗血管生成相關(guān)療法。
這些技術(shù)已被證實適于檢測一系列關(guān)鍵大分子物質(zhì)，包括cAMP、色氨酸、S-腺苷蛋氨酸、磷酸二酯酶、生物素、胰島素、C肽、白蛋白、C反應蛋白、IgG、IgM和大腸桿菌等等。
Mediomics相關(guān)產(chǎn)品
| Product | Catalog Number | Kit components |
| Bridge-It Assay Kits | ||
| Bridge-It cAMP 384 | 122938 | 10uM cAMP stock standard in H2O, 2X Assay Solution A, 10X Lysis Buffer, Buffer B |
| Bridge-It cAMP 100 | 122939 | 10uM cAMP stock standard in H2O, 2X Assay Solution A, 10X Lysis Buffer, Buffer B |
| Bridge-It cAMP 96 | 122935 | 10uM cAMP stock standard in H2O, Assay Solution A, 10X Lysis Buffer, Buffer B, 10X KRB-IBMX Buffer |
| Bridge-It cAMP 50 | 122934 | 10uM cAMP stock standard in H2O, Assay Solution A, 10X Lysis Buffer, Buffer B, 10X KRB-IBMX Buffer |
| Bridge-It cAMP-PDE 384 | PD1007-384 | 10uM cAMP stock standard, 2X Assay Solution, Stop Solution, 10X PDE Reaction Buffer, Buffer B |
| Bridge-It cAMP-PDE 100 | PD1007-100 | 10uM cAMP stock standard, 2X Assay Solution, Stop Solution, 10X PDE Reaction Buffer, Buffer B |
| Bridge-It cAMP-PDE 96 | PD1016-96 | 10uM cAMP stock standard, 2X Assay Solution, Stop Solution, 10X PDE Reaction Buffer, Buffer B |
| Bridge-It cAMP-PDE 60 | PD1016-60 | 10uM cAMP stock standard, 2X Assay Solution, Stop Solution, 10X PDE Reaction Buffer, Buffer B |
| Bridge-It SAM 384 | 1-1-1004B | 10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM |
| Bridge-It SAM 100 | 1-1-1004A | 10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM |
| Bridge-It SAM 96 | 1-1-1003B | 10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM |
| Bridge-It SAM 50 | 1-1-1003A | 10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM |
| Bridge-It L-MET 384 | 1-1-1006B | L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution |
| Bridge-It L-MET 100 | 1-1-1006A | L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution |
| Bridge-It L-MET 96 | 1-1-1005B | L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution |
| Bridge-It L-MET 50 | 1-1-1005A | L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution |
| Bridge-It L-Tryptophan 384 | 1-1-1002B | 1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C |
| Bridge-It L-Tryptophan 100 | 1-1-1002A | 1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C |
| Bridge-It L-Tryptophan 96 | 1-1-1001B | 1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C |
| Bridge-It L-Tryptophan 50 | 1-1-1001A | 1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C |
| TR-FRET Bridge-It Assay Kits | ||
| TR-FRET Bridge-It® L-Tryptophan 100 | TRF-1-1-1002A | 1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C |
| TR-FRET Bridge-It® L-Tryptophan 384 | TRF-1-1-1002B | 1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C |
| TR-FRET Bridge-It® L-Tryptophan 50 | TRF-1-1-1001A | 1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C |
| TR-FRET Bridge-It® L-Tryptophan 96 | TRF-1-1-1001B | 1 mM L-tryptophan standard in H20, L-Tryptophan Assay Solution A, Buffer W, Buffer C |
| TR-FRET Bridge-It® SAM 100 | TRF-1-1-1004A | 10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM |
| TR-FRET Bridge-It® SAM 384 | TRF-1-1-1004B | 10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM |
| TR-FRET Bridge-It® SAM 50 | TRF-1-1-1003A | 10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM |
| TR-FRET Bridge-It® SAM 96 | TRF-1-1-1003B | 10 mM S-adenosyl methionine standard in 10mM 2-mercaptoethanol, SAM Assay Solution, Buffer S, Buffer CM |
| TR-FRET Bridge-It® L-MET 50 | TRF-1-1-1005A | L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution |
| TR-FRET Bridge-It® L-MET 96 | TRF-1-1-1005B | L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution |
| TR-FRET Bridge-It® L-MET 100 | TRF-1-1-1006A | L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution |
| TR-FRET Bridge-It® L-MET 384 | TRF-1-1-1006B | L-Methionine Standard (3.2 mM), Enzyme Solution L, Buffer L, SAM Assay Solution |
| FRET-PINCER Assay Kits | ||
| hAlbumin FRET 384 | AH1005-384 | Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hAlbumin FRET 100 | AH1005-100 | Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hAlbumin FRET 50 | AH1005-50 | Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hAlbumin FRET 96 | AH1005-96 | Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hCRP FRET 384 | CH1008-384 | hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hCRP FRET 100 | CH1008-100 | hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hCRP FRET 96 | CH1021-96 | hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hCRP FRET 50 | CH1021-50 | hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hInsulin FRET 384 | IH1010-384 | Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hInsulin FRET 100 | IH1010-100 | Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hInsulin FRET 50 | IH1017-50 | Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hInsulin FRET 96 | IH1017-96 | Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| Mouse IgM FRET 384 | IM1009-384 | Mouse IgM standard (2 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| Mouse IgM FRET 100 | IM1009-100 | Mouse IgM standard (2 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| Mouse IgG FRET 384 | MIG1012-384 | Mouse IgG standard (2 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| Mouse IgG FRET 100 | MIG1012-100 | Mouse IgG standard (2 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| rIgG FRET 96 | RI1024-96 | Rabbit IgG standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| rIgG FRET 50 | RI1024-50 | Rabbit IgG standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| rIgG FRET 384 | RI1015-384 | Rabbit IgG standard (2 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| rIgG FRET 100 | RI1015-100 | Rabbit IgG standard (2 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| TRF-PINCER Assay Kits | ||
| Biotin TRF 384 | BTTR-1006-384 | Biotin standard (2 uM), PINCER A, PINCER B, Assay Solution C, Buffer M |
| Biotin TRF 100 | BTTR-1006-100 | Biotin standard (2 uM), PINCER A, PINCER B, Assay Solution C, Buffer M |
| hAlbumin TRF 384 | AHTR1005-384 | Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hAlbumin TRF 100 | AHTR1005-100 | Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hAlbumin TRF 50 | AHTR1005-50 | Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hAlbumin TRF 96 | AHTR1005-96 | Human albumin standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hCRP TRF 50 | CHTR1021-50 | hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hCRP TRF 96 | CHTR1021-96 | hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hCRP TRF 100 | CHTR1008-100 | hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hCRP TRF 384 | CHTR1008-384 | hCRP standard (1.6 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hIgG TRF 384 | HITR1011-384 | hIgG standard (40 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hIgG TRF 100 | HITR1011-100 | hIgG standard (40 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hInsulin TRF 384 | IHTR1010-384 | Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hInsulin TRF 100 | IHTR1010-100 | Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hInsulin TRF 50 | IHTR1017-50 | Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| hInsulin TRF 96 | IHTR1017-96 | Insulin standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| mIgG TRF 384 | MIGTR1012-384 | Mouse IgG standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| mIgG TRF 200 | MIGTR1012-200 | Mouse IgG standard (8 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| mIgG TRF 96 | MIGTR1020-96 | Mouse IgG standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| mIgG TRF 50 | MIGTR1020-50 | Mouse IgG standard (5.12 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| mIgM TRF 384 | IMTR1009-384 | Mouse IgM standard (80 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| mIgM TRF 200 | IMTR1009-200 | Mouse IgM standard (80 ug/ml), PINCER A, PINCER B, Buffer C, Buffer M |
| Other Products | ||
| Recombinant human methionine aminopeptidase-2 | PM-803262-100 µg | Each vial contains 200 µl (~100 µg) of purified human methionine aminopeptidase-2 in 20 mM Hepes, pH 7.4, 0.15 M NaCl, 0.02% NaN3, 10% glycerol and 0.1 mM Co(II) |
| Anti-recombinant human MetAP-2 (full-length) Polyclonal Antibody | PAB-503208 | Each vial contains 120 µl of anti-rhMetAP2 IgG (0.85 mg/ml) in TBS with 0.02% NaN3 |
| Anti-hMetAP-2 (C-terminal) Polyclonal Antibody | PAB-503209 | Each vial contains 100 µl of anti-hMetAP2 IgG in PBS |
| 10X KRB-IBMX Buffer | 122937 | 10X KRB-IBMX Buffer solution is a mixture of a 10X concentrate of Krebs-Ringer Bicarbonate Buffer (KRB) supplemented with a 10X concentration (i.e., 7.5 mM) of 3-Isobutyl-1-Methylxanthine (IBMX) and containing 10% ethanol (for use in cAMP Assays, order with cAMP assay kits) |
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